Selective in vivo rescue by GroEL/ES of thermolabile folding intermediates to phage P22 structural proteins.
نویسندگان
چکیده
منابع مشابه
Thermolabile folding intermediates: inclusion body precursors and chaperonin substrates.
An unexpected aspect of the expression of cloned genes is the frequent failure of newly synthesized polypeptide chains to reach their native state, accumulating instead as insoluble inclusion bodies. Amyloid deposits represent a related state associated with a variety of human diseases. The critical folding intermediates at the juncture of productive folding and the off-pathway aggregation reac...
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Cytochrome-c (cyt-c) is an electron transport protein, and it is present throughout the evolution. More than 280 sequences have been reported in the protein sequence database (www.uniprot.org). Though sequentially diverse, cyt-c has essentially retained its tertiary structure or fold. Thus a vast data set of varied sequences with retention of similar structure and fun...
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Two intermediates in the synthesis and maturation of phage P22 DNA have been identified by sedimentation of lysozymedetergent lysates of induced lysogens or infected cells through sucrose density-gradients. Intermediate I contains most of the radioactivity incorporated during a short pulse of [3H]thymidine ; it sediments at a rate in excess of 1000 s. If a short pulse of [3H]thymidine is chsaed...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)46878-0